The next-generation organic fluorophores offered by Lumidyne Technologies are designed and custom tailored to enable cutting edge research in the biological sciences. These advances have led to a broad range of new insights into the mechanisms of macromolecular behaviors.

1: Zheng Q, Jockusch S,  Zhou Z, Altman RB, Zhao H, Asher W, Holsey M, Mathiasen S, Geggier P, Javitch JA, Blanchard SC. Electronic tuning of self-healing fluorophores for live-cell and single-molecule imaging. Chem Sci. 2017 Jan 1;8(1):755-762. doi: 10.1039/c6sc02976k. ePub 2016 Sept 7. PubMed PMID: 28377799.

2: Dyla M Terry DS, Kjaergaard M, Sørensen TL, Lauwring Andersen J, Andersen JP, Rohde Knudsen C, Altman RB, Nissen P, Blanchard SC. Dynamics of P-type ATPase transport revealed by single-molecule FRET. Nature. 2017 Nov 16;551(7680):346-351. doi: 10.1038/nature24296. Epub 2017 Nov 8. PubMed PMID:29144454.

3: Alejo JL, Blanchard SC. Miscoding-induced stalling of substrate translocation on the bacterial ribosome. Proc Natl Acad Sci U S A. 2017 Oct10;114(41):E8603-E8610. doi: 10.1073/pnas.1707539114. Epub 2017 Sep 25. PubMed PMID: 28973849; PubMed Central PMCID: PMC5642701.

4: Gregorio GG, Masureel M, Hilger D, Terry DS, Juette M, Zhao H, Zhou Z, Perez-Aguilar JM, Hauge M, Mathiasen S, Javitch JA, Weinstein H, Kobilka BK, Blanchard SC. Single-molecule analysis of ligand efficacy in β(2)AR-G-protein activation. Nature. 2017 Jul 6;547(7661):68-73. doi: 10.1038/nature22354. Epub2017 Jun 7. PubMed PMID: 28607487; PubMed Central PMCID: PMC5502743.

5: Vušurović N, Altman RB, Terry DS, Micura R, Blanchard SC. Pseudoknot Formation Seeds the Twister Ribozyme Cleavage Reaction Coordinate. J Am Chem Soc. 2017 Jun 21;139(24):8186-8193. doi: 10.1021/jacs.7b01549. Epub 2017 Jun 9. PubMed PMID: 28598157; PubMed Central PMCID: PMC5697751.

6: Lv Z, Krasnoslobodtsev AV, Zhang Y, Ysselstein D, Rochet JC, Blanchard SC, Lyubchenko YL. Effect of acidic pH on the stability of α-synuclein dimers. Biopolymers. 2016 Oct;105(10):715-24. doi: 10.1002/bip.22874. PubMed PMID: 27177831; PubMed Central PMCID: PMC4958566.

7: Wasserman MR, Alejo JL, Altman RB, Blanchard SC. Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation. Nat Struct Mol Biol. 2016 Apr;23(4):333-41. doi: 10.1038/nsmb.3177. Epub 2016 Feb 29. PubMed PMID: 26926435; PubMed Central PMCID: PMC4821728.

8: Juette MF, Terry DS, Wasserman MR, Altman RB, Zhou Z, Zhao H, Blanchard SC. Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale. Nat Methods. 2016 Apr;13(4):341-4. doi: 10.1038/nmeth.3769. Epub 2016 Feb 15. PubMed PMID: 26878382; PubMed Central PMCID: PMC4814340.

9: Zheng Q, Jockusch S, Rodríguez-Calero GG, Zhou Z, Zhao H, Altman RB, Abruña HD, Blanchard SC. Intra-molecular triplet energy transfer is a general approach to improve organic fluorophore photostability. Photochem Photobiol Sci. 2016 Feb;15(2):196-203. doi: 10.1039/c5pp00400d. Epub 2015 Dec 23. PubMed PMID: 26700693; PubMed Central PMCID: PMC4749434.

10: Akyuz N, Georgieva ER, Zhou Z, Stolzenberg S, Cuendet MA, Khelashvili G, Altman RB, Terry DS, Freed JH, Weinstein H, Boudker O, Blanchard SC. Transport domain unlocking sets the uptake rate of an aspartate transporter. Nature. 2015 Feb 5;518(7537):68-73. doi: 10.1038/nature14158. PubMed PMID: 25652997; PubMed Central PMCID: PMC4351760.

11: Olivier NB, Altman RB, Noeske J, Basarab GS, Code E, Ferguson AD, Gao N, Huang J, Juette MF, Livchak S, Miller MD, Prince DB, Cate JH, Buurman ET, Blanchard SC. Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome. Proc Natl Acad Sci USA. 2014 Nov 18;111(46):16274-9. doi: 10.1073/pnas.1414401111. Epub 2014 Nov 3. PubMed PMID: 25368144; PubMed Central PMCID: PMC4246262.
 
12: Munro JB, Gorman J, Ma X, Zhou Z, Arthos J, Burton DR, Koff WC, Courter JR, Smith AB 3rd, Kwong PD, Blanchard SC, Mothes W. Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions. Science. 2014 Nov 7;346(6210):759-63. doi: 10.1126/science.1254426. Epub 2014 Oct 8. PubMed PMID: 25298114; PubMed Central PMCID: PMC4304640.
 
13: Pancera M, Zhou T, Druz A, Georgiev IS, Soto C, Gorman J, Huang J, Acharya P, Chuang GY, Ofek G, Stewart-Jones GB, Stuckey J, Bailer RT, Joyce MG, Louder MK, Tumba N, Yang Y, Zhang B, Cohen MS, Haynes  BF, Mascola JR, Morris L, Munro JB, Blanchard SC, Mothes W, Connors M, Kwong PD. Structure and immune recognition of trimeric pre-fusion HIV-1 Env. Nature. 2014 Oct 23;514(7523):455-61. doi: 10.1038/nature13808. Epub 2014 Oct 8. PubMed PMID: 25296255; PubMed Central PMCID: PMC4348022.
 
14: Burnett BJ, Altman RB, Ferguson A, Wasserman MR, Zhou Z, Blanchard SC. Direct evidence of an elongation factor-Tu/Ts·GTP·Aminoacyl-tRNA quaternary complex. J Biol Chem. 2014 Aug 22;289(34):23917-27. doi: 10.1074/jbc.M114.583385. Epub 2014 Jul 2. PubMed PMID: 24990941; PubMed Central PMCID: PMC4156062.
 
15: Juette MF, Terry DS, Wasserman MR, Zhou Z, Altman RB, Zheng Q, Blanchard SC. The bright future of single-molecule fluorescence imaging. Curr Opin Chem Biol. 2014 Jun;20:103-11. doi: 10.1016/j.cbpa.2014.05.010. Epub 2014 Jun 21. Review. PubMed PMID: 24956235; PubMed Central PMCID: PMC4123530.
 
16: Zheng Q, Jockusch S, Zhou Z, Blanchard SC. The contribution of reactive oxygen species to the photobleaching of organic fluorophores. Photochem Photobiol. 2014 Mar-Apr;90(2):448-54. doi: 10.1111/php.12204. Epub 2013 Dec 2. PubMed PMID: 24188468; PubMed Central PMCID: PMC3959289.
 
17: Zheng Q, Juette MF, Jockusch S, Wasserman MR, Zhou Z, Altman RB, Blanchard SC. Ultra-stable organic fluorophores for single-molecule research. Chem Soc Rev. 2014 Feb 21;43(4):1044-56. doi:  0.1039/c3cs60237k. Review. PubMed PMID: 24177677; PubMed Central PMCID: PMC3946787.
 
18: Alejo JL, Blanchard SC, Andersen OS. Small-molecule photostabilizing agents are modifiers of lipid bilayer properties. Biophys J. 2013 Jun 4;104(11):2410-8. doi: 10.1016/j.bpj.2013.04.039. PubMed PMID: 23746513; PubMed Central PMCID: PMC3672891.
 
19: Zheng Q, Jockusch S, Zhou Z, Altman RB, Warren JD, Turro NJ, Blanchard SC. On the Mechanisms of Cyanine Fluorophore Photostabilization. J Phys Chem Lett. 2012 Aug 16;3(16):2200-2203. Epub 2012 Jul 30.  PubMed PMID: 22984636; PubMed Central PMCID: PMC3439216.
 
20: Altman RB, Zheng Q, Zhou Z, Terry DS, Warren JD, Blanchard SC. Enhanced photostability of cyanine fluorophores across the visible spectrum. Nat Methods. 2012 Apr 27;9(5):428-9. doi: 10.1038/nmeth.1988. Erratum in: Nat Methods. 2012 Jun;9(6):626. PubMed PMID: 22543373; PubMed Central PMCID: PMC3574820.
 
21: Altman RB, Terry DS, Zhou Z, Zheng Q, Geggier P, Kolster RA, Zhao Y, Javitch JA, Warren JD, Blanchard SC. Cyanine fluorophore derivatives with enhanced photostability. Nat Methods. 2011 Nov 13;9(1):68-71. doi: 10.1038/nmeth.1774. PubMed PMID: 22081126; PubMed Central PMCID: PMC3433158.
 
22: Dave R, Terry DS, Munro JB, Blanchard SC. Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging. Biophys J. 2009 Mar 18;96(6):2371-81. doi: 10.1016/j.bpj.2008.11.061. PubMed PMID: 19289062; PubMed Central PMCID: PMC2907709.